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. 2020 Oct 16;10:506959. doi: 10.3389/fonc.2020.506959

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Copyright © 2020 Quintero Escobar, Costa, Martins, Costa, vanHelvoort Lengert, Boldrini, Morini da Silva, Lopes, Vidal, Krepischi, Maschietto and Tasic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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¹H-NMR mean spectra of osteosarcoma patients (in red) and healthy controls (in blue) acquired using CPMG (cpmgpr1d) pulse sequence. Main signals have been assigned: 1. Fatty acyls' -CH₃; 2. Valine (Val); 3. Fatty acyls' -CH₂- hydrogens as in CH₃(CH₂)_n; 4. Lactate (Lac); 5. Alanine (Ala); 6. Fatty acyls' from -CH₂- group next to carboxyl group as in CH₂CH₂C(O); 7. Fatty acyls' CH₂CH=; 8. Glutamine (Gln); 9. Glucose (Glc); (*glucose resonance hydrogens); 10. Fatty acyls' -CH=CH-; 11. tyrosine (Tyr); 12. histidine (His); 13. phenylalanine (Phe); 14. formate (For). Excluded spectral regions are indicated with the dotted lines.