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. 2020 Oct 26;12(10):1152–1170. doi: 10.4252/wjsc.v12.i10.1152

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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Neural/glial antigen 2 immunoreactivity (green fluorescence) evaluated after one day (left column, 1 d), three days (middle column, 3 d) and six days (right column, 6 d) of cell growth. First row: Human adipose-derived mesenchymal stem cells (hASCs) cultured in basal medium; Second row: hASCs in basal medium stimulated with transforming growth factor; Third row: hASCs cultured in pericyte medium without pericyte growth supplement; Fourth Row: hASCs cultured in complete pericyte medium; Fifth row: Human retinal pericyte cells cultured in complete pericyte medium (hRPCs). Photomicrographs show that clear neural/glial antigen 2 immunoreactivity is present only in hASCs pre-cultured in complete pericyte medium and human retinal pericyte cells (last two rows). The immunostaining detected at day 1 is similar to that observed at day 3 and 6. Very weak immunostaining is visible in the other three hASC groups (first three rows). Blue fluorescence indicates DAPI staining of cell nuclei. Scale bar: 50 μm.