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. 2020 Oct 29;11:5466. doi: 10.1038/s41467-020-19278-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Normalized average microarray signal intensities detected upon binding of the GST-tagged MORC4 CW domain to the peptides carrying indicated PTMs. Data are represented as mean values +/− S.D. from n ≥ 3 independent arrays. See also Supplementary Data 1. b Cartoon representation of CW in complex with histone H3K4me3 tail. c Peptide pull-down assays of the GST-tagged MORC4 CW domain using the indicated histone H3 peptides. Images are representative of three independent experiments with similar results (n = 3). Source data are provided in a Source Data file. d, e Superimposed ¹H,¹⁵N HSQC spectra of the ¹⁵N-labeled WT MORC4 CW domain collected upon titration with the histone H3K4me3 (d) and unmodified H3 (e) peptides. Spectra are color coded according to the protein-to-peptide molar ratio. f Representative binding curve used to determine K_d values by tryptophan fluorescence. g Binding affinities of MORC4 CW for the indicated histone H3 peptides measured by tryptophan fluorescence. Data are represented as mean values +/− S.D. from three independent experiments (n = 3). Source data are provided in a Source Data file.