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. 2020 Oct 29;11:5466. doi: 10.1038/s41467-020-19278-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Superimposed ¹H,¹⁵N HSQC spectra of the ¹⁵N-labeled MORC4 CW domain collected upon titration with the unlabeled ATPase domain of MORC4. b Superimposed ¹H,¹⁵N HSQC spectra of the ¹⁵N-labeled MORC4 CW domain (in complex with H3 peptide) collected upon titration with the unlabeled ATPase domain. Spectra in (a, b) are color coded according to the protein-to-ligands molar ratio. (c, d) EMSA with 601 DNA in the presence of increasing amounts of MORC4 CW (c) and MORC3 CW (d). e Cartoon representation of MORC4 CW in complex with histone H3K4me3 tail and DNA. f, g Electrostatic surface potential of the CW domain of MORC4 (f) and MORC3 (g) (within the ATPase-CW cassette) was generated using APBS in Pymol with a range of –5/5 kT/e and colored blue and red for positive and negative charges, respectively. The ATPase domain is shown as ribbon and colored green and white in MORC4 and MORC3, respectively. The missing loops (residues K441 and R463) in the model of MORC4 CW were completed for this figure. h EMSA with 601 DNA in the presence of increasing amounts of mutant MORC4 CW_KRR. i Superimposed ¹H,¹⁵N HSQC spectra of the ¹⁵N-labeled MORC4 CW_KRR domain collected upon titration with the histone H3K4me3 peptide. Spectra are color coded according to the protein-to-peptide molar ratio.