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. 2020 Oct 29;10:18565. doi: 10.1038/s41598-020-75210-6

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

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Treatment schema. Treatment protocol. Pioglitazone (30 mg/kg weight) was administered orally 1.5 h before and 2 h after the initiation of ischemia. SL4-RFP cells 1 × 10⁶ was injected into the spleen. After 15 min, the hepatoduodenal ligament was clamped with a vessel clip, which was released after 5 min. After the procedure, Pioglitazone was orally administrated daily to mice from day 0–21.