Figure 2. Effects of blocking IL1α and/or IL1β on tumor growth.

2×10⁵ EL-4 cells were injected i.d. into mice. (A) WT mice were treated with anti-IL1β, anti-IL1α or both 2 days before and then every 2–3 days after tumor cell injection. Mice injected with control Ab were used as controls (n=5–6 mice per group). (B) IL1R1^−/− mice and IL1α^−/− mice were treated with anti-IL1β or control Ab 2 days before and then every 2–3 days after tumor cell injection (n=5–8 mice per group). (C) IL1β^−/− mice were treated with anti-IL1α or control Ab. Tumor size and survival was recorded every 2–3 days and plotted (n=5–8 mice per group). Statistics were generated compared to IL1β^−/− mice with control Ab. Graphs show mean ± SEM. *P<0.05, **P<0.01, ***P<0.001. NS=not significant.