. 2020 Oct 29;10:18658. doi: 10.1038/s41598-020-75627-z

Table 2.

Photo-CIDNP experimental results and obtained ¹⁵N chemical shifts from the eight LOV proteins studied in this work.

Mutants	$r_{FW}$ . (Å)	¹⁵N photo-CIDNP	¹H photo-CIDNP	¹⁵N chemical shift (ppm)
Mutants	$r_{FW}$ . (Å)	¹⁵N photo-CIDNP	¹H photo-CIDNP	FMN N-5	FMN-10	Trp N-1
CrPhot-LOV1-C57S	~ 11	+	+	344.1	155.0	127.6
PtAureo1a-LOV-C287S	~ 11	+	+	n.a.	n.a.	127.8
Mr4511-C71S-F130W	~ 6	–	–	n.a.	n.a.	n.a.
Mr4511-C71S-Y116W	~ 9	+	+	345.1	156.2	127.7
Mr4511-C71S-Q112W	~ 11	+	+	345.3	156.4	127.2
Mr4511-C71S-Y129W	~ 11	+	+	345.5	156.2	127.7
Mr4511-C71S-K57W	~ 17	+ (new)	+ (new)	345.2	n.a.	n.a.
Mr4511-C71S	No Trp	+ (new)	+ (new)	345.3	n.a.	n.a.

Trp tryptophan, + tryptophan-derived photo-CIDNP effect, + (new) non-tryptophan-derived photo-CIDNP effect, – no photo-CIDNP effect, n.a. not available.