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. 2020 Oct 29;10:18590. doi: 10.1038/s41598-020-75344-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Calcium imaging of gnrh2-expressing chemosensory neurons. Fluorescence images of Ciona larvae expressing G-CaMP8 in the chemosensory aATENs. (a) The putative sensory cilum of each aATEN was labeled with G-CaMP8 fluorescence (dotted circle). (b, c) An example of a larva showing dynamic Ca²⁺ transients in a pair of aATENs (arrowheads) at 19 hpf. (c) Representative images of the larva recorded at the times indicated (in seconds). Serial images of the larva shown in (b, c) are shown in Movie S1. Quantitative analysis of the imaging data is shown in Supplementary Figure S6.