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. 2020 Oct 16;10:575711. doi: 10.3389/fonc.2020.575711

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Copyright © 2020 Wang, Guo, Zhang, Li, Xu, Li, Wang and Zhan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Combination of mirin and NU7441 with ionizing radiation (IR) treatment significantly enhanced double-strand breaks (DSBs) in esophageal squamous cell cancer (ESCC) cells. (A, B) DSBs were indicated by immunostaining with γ-H2AX. Combinations of mirin (50 µM) or/and NU7441 (5 µM) with IR (6 Gy) treatment significantly improved the number of γ-H2AX foci in both YES2 and KYSE30 cells. Scale bar = 30 µm. All the experiments were independently performed in triplicate. The error bars represent the standard deviation and P values were evaluated by Student’s t-test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, n.s. P > 0.05.