(a) Fluorogenic conversion of APE to Pittsburgh Green. (b) Fluorogenic
conversion of PPE to Pittsburgh Green. (c) The heat map of the metal
vs ligand screening for the conversion of APE to Pittsburgh Green.
(d) The heat map of the metal vs ligand screening for the conversion
of PPE to Pittsburgh Green. For both heat maps, the colors indicate
relative fluorescence intensities. Conditions: 1 μM metal, 20
μM APE or PPE, 200 μM ligand, 1:9 (v/v) DMSO/1.2 M phosphate
buffer (pH 7.0), 25 °C, 2 h, n = 1. Row 37 contains
no ligand. See Tables S1 and S2 for quantitative
data. (e) Ligands used for the screenings.