Figure 8.

(a) Kinetic study to determine turnover frequency. Conditions: 200 nM CuSO₄, 20 μM PPE, 160 μM DMAPPP, 15:85 (v/v) EtOH/500 mM borate buffer, pH 8.5, 30 °C, n = 3. (b) Copper concentration dependence to determine the limit of detection and limit of quantification. Conditions: 20 μM PPE, 100 μM DMAPPP, 15:85 (v/v) EtOH/50 mM borate buffer, pH 8.5, 2 h, 25 °C, n = 8 for plate reader, n = 4 for fluorometer. (c) Visualization of the relative concentrations of copper in drinking water samples. (d) Structures of compounds used in (e) and (f). (e, f) Quantification of copper in drug-like samples. Conditions: 30 ppm (e) or 300 ppm (f) Cu(II) in the solid phase, 20 μM PPE, 160 μM DMAPPP, 14.2:3.3:85 (v/v/v) EtOH/DMSO/500 mM borate buffer, pH 8.5, 24 °C, 1 h, n = 3. (g) Detection of copper in SOD. Conditions: 0 or 0.5 mg/mL SOD, 0.5 μM PPE, 10 μM DMAPPP, 0.5:0.5:99 EtOH/DMSO/500 mM borate buffer, pH 8.5, 25 °C, n = 2. (h) Distribution of copper between water and CHCl₃ in the presence of DMAPPP. Partition conditions: CHCl₃ and aqueous, pH 7 buffer, 0 or 5 μM CuSO₄ in H₂O. Analysis by fluorescence: 20 μM PPE, 160 μM DMAPPP, 15:85 (v/v) EtOH/500 mM borate buffer, pH 8.5, 1 h, 25 °C, n = 3.