Table 1.
Microbiological and molecular characteristics of the CMY-producing E. coli isolates
| Key | MIC (μg/mL) | CAZ CLOX |
CAZ BOR |
AmpC type | ESBL test | Other enzymes | PFGE | ST131-O25b clone | Phylogroup | MLST | CC | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CRO | CAZ | FEP | FOX | IMP | MEM | ETP | |||||||||||
| Ec 24 | > 32 | ≥ 16 | ≥ 16 | 128 | ≤ 1 | ≤ 1 | 1 | − | + | CMY-2 | + | CTX-M-15 | NT | − | D | ST405 | 405 |
| Ec 35 | > 32 | ≥ 16 | ≥ 16 | > 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | − | + | CMY-2 | + | CTX-M-79 | NR | − | F | ST457 | ND |
| Ec 41 | > 32 | ≥ 16 | ≥ 16 | 64 | ≤ 1 | ≤ 1 | ≤ 0.5 | − | − | CMY-2 | + | TEM-1, CTX-M-15 | NR | − | F | ST648 | 648 |
| Ec 55 | > 32 | ≥ 16 | ≥ 16 | > 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-2 | + | CTX-M-15 | NR | − | A | ST10 | 10 |
| Ec 65 | > 32 | ≥ 16 | ≥ 16 | 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-2 | + | CTX-M-15 | NR | − | B2 | ST12 | 12 |
| Ec 109 | > 32 | ≥ 16 | ≥ 16 | 16 | ≤ 1 | ≤ 1 | ≤ 0.5 | − | − | CMY-2 | + | CTX-M-15 | NR | − | A | ST10 | 10 |
| Ec 223 | > 32 | 8 | ≥ 16 | 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | − | + | CMY-2 | + | CTX-M-15 | NR | + | B2 | ST131 | ND |
| Ec 278 | > 32 | ≥ 16 | ≤ 2 | 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-2 | − | Absent | NR | − | A | ST168 | 168 |
| Ec 394 | > 32 | ≥ 16 | ≥ 16 | > 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-42 | − | TEM-190 | NR | − | A | ST1284 | 10 |
| Ec 445 | > 32 | ≥ 16 | ≥ 16 | 128 | ≥ 4 | ≥ 4 | ≥ 2 | − | − | CMY-2 | + | CTX-M-15, OXA-48 | NR | − | A | ST10 | 10 |
| Ec 480 | > 32 | ≥ 16 | ≥ 16 | > 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-42 | − | Absent | NT | − | A | ST361 | ND |
| Ec 481 | > 32 | ≥ 16 | ≥ 16 | 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-42 | + | TEM-190 | NR | − | C | ST23 | 23 |
| Ec 599 | 16 | ≥ 16 | ≤ 2 | 64 | ≤ 1 | ≤ 1 | ≤ 0.5 | − | + | CMY-2 | − | TEM-1 | NR | − | A | ST101 | 101 |
| Ec 668 | > 32 | ≥ 16 | ≥ 16 | 128 | ≤ 1 | ≤ 1 | 1 | + | + | CMY-42 | + | CTX-M-15 | NR | − | A | ST10 | 10 |
| Ec 678 | 8 | 8 | ≤ 2 | 128 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-2 | − | Absent | NR | − | E | ST350 | 350 |
| Ec 686 | > 32 | ≥ 16 | ≥ 16 | 128 | ≤ 1 | ≤ 1 | 1 | + | + | CMY-2 | + | CTX-M-15 | NR | − | D | ST69 | 69 |
| Ec 689 | 8 | 8 | ≤ 2 | 64 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-2 | − | TEM-1 | NR | − | A | ND | ND |
| Ec 714 | > 32 | ≥ 16 | ≥ 16 | 64 | ≤ 1 | ≤ 1 | ≤ 0.5 | + | + | CMY-2 | + | CTX-M-15 | NR | − | Clade I | ND | ND |
In bold are the high-risk clone
MIC minimum inhibitory concentration, CRO ceftriaxone, CAZ ceftazidime, FEP cefepime, FOX cefoxitin, IMP imipenem, MEM meropenem, ETP ertapenem, CLOX cloxacillin, BOR boronic acid, ESBL extended-spectrum beta-lactamases, PFGE pulsed-field gel electrophoresis, MLST multilocus sequence typing, CC clonal complex, NT non-typeable, ND not determined, NR not related