Table 1.
Primer, sequences, and protocols for quantitative real-time polymerase chain reaction
| Primer | Sequenzen (5′-3′) | Primer concentration (nM) | Amplification | Efficiency (E = 10−1/slope) |
|---|---|---|---|---|
| ALP |
TCAAGGGTCAGCTCCACCACA ATTGGCCTTCACCCCACACA |
300 | 95 °C 10 s, 60 °C 10 s, 72 °C 30 s for 45 cycles | 2.01 |
| Collagen 1A1 |
GCTTCCCTGGTCTTCCTG TCTCACCACGGTCACCCT |
500 | 95 °C 10 s, 65 °C 10 s, 72 °C 15 s for 40 cycles | 2.01 |
| GAPDH |
TGCACCACCAACTGCTTAGC GGCATGGACTGTGGTCATGAG |
300 | 95 °C 10 s, 60 °C 10 s, 72 °C 15 s for 40 cycles | 2.04 |
| Cathepsin K |
TTCCCGCAGTAATGACACC TTTCCCCAGTTTTCTCCCC |
500 | 95 °C 10 s, 63 °C 10 s, 72 °C 20 s for 40 cycles | 1.83 |
| M-CSF |
CCGAGGAGGTGTCGGAGTAC AATTTGGCACGAGGTCTCCAT |
300 | 95 °C 10 s, 60 °C 10 s, 72 °C 15 s for 40 cycles | 2.01 |
| MMP 13 |
GACTTCACGATGGCATTGCTG GCATCAACCTGCTGAGGATGC |
300 | 95 °C 10 s, 62 °C 10 s, 72 °C 20 s for 40 cycles | 1.90 |
| OPG |
CTGCGCGCTCGTGTTTC ACAGCTGATGAGAGGTTTCTTCGT |
300 | 95 °C 30 s, 60 °C 60 s, 72 °C 15 s for 40 cycles | 1.99 |
| RANK-L |
CATCCCATCTGGTTCCCATAA GCCCAACCCCGATCATG |
300 | 95 °C 10 s, 60 °C 10 s, 72 °C 15 s for 40 cycles | 2.07 |
| TNF α |
CCCAGGGACCTCTCTCTAATC GCTTGAGGGTTTGCTACAACATG |
300 | 95 °C 30 s, 60 °C 60 s, 72 °C 15 s for 40 cycles | 1.97 |