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. 2020 Oct 29;39:229. doi: 10.1186/s13046-020-01748-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — UPK1A-AS1 interacts with EZH2. a. UPK1A-AS1 cytosolic and nuclear expression levels in SK-Hep-1 and MHCC-97H cells. β-Actin and U6 were used as cytosolic and nuclear markers, respectively. Both NEAT1 and MALAT1 were localized to the nucleus. b-c. RIP assay was performed in MHCC-97H cells and the co-precipitated RNA was subjected to qRT-PCR for UPK1A-AS1(^***P < 0.001). d. RNA pull-down assay was carried out to confirm the association between UPK1A-AS1 and EZH2. e. Effect of UPK1A-AS1 overexpression on EZH2 and H3K27M3 expressions was measured by western blotting. f. Correlation between UPK1A-AS1 and EZH2 was analyzed in HCC samples from TCGA dataset. g. EZH2 expression level in the cytoplasm or nucleus of MHCC-97H cells. β-Actin was used as a cytosol marker, LaminB1 served as a nuclear marker. h. Translocation of EZH2 from the cytoplasm to the nucleus was detected by immunofluorescence assay. i. Immunoprecipitation assay identified the increased interaction between EZH2 and SUZ12 in UPK1A-AS1-overexpressing MHCC-97H cells. β-Actin was used as negative control