Fig. 5.

Effect of adalimumab on TNFα-induced genes modulated by IL6/sIL6R. a Schematic representation of the experiment. SF were stimulated with TNFα (10 ng/ml) for 24 h. Inflammatory input was removed by washing cells and the second stimuli IL6/sIL6R (50 ng/ml each) was added in the presence of adalimumab (ADA) (10 μg/ml) to block any residual TNFα. b Graphics show the change on the mRNA expression of representative genes. Untreated SF was used as reference. Data are mean ± SEM of three independent experiments