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. 2020 Oct 29;18:156. doi: 10.1186/s12915-020-00888-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Fluorescence microscopy of yeast cells expressing EGFP-labeled segments of AAC. a Upper panel: Images of yeast cells expressing wild type AAC (AAC_WT, ADP/ATP carrier of Neurospora crassa) containing an EGFP moiety at the C-terminus. Second panel: Cells expressing AAC_Δpos (all positively charged residues of the matrix loops exchanged against glycine). Third panel: AAC module III (residues 221–313). Lower panel: AAC module III_Δpos (the positively charged residues of the matrix loop exchanged against glycine). MitoTracker Orange was used for labelling of the mitochondria. Scale bars, 2 μm. b Yeast cells expressing EGFP-labeled segments of AAC module III. c Amino acid sequence of Neurospora crassa AAC residues 238–305. The assignments are based on the crystal structure of bovine ADP/ATP carrier [1, 40]. Red, positively charged residues; blue, negatively charged residues