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. 2020 Oct 30;20:275. doi: 10.1186/s12871-020-01179-4

Fig. 2.

Fig. 2

Bupivacaine restrains the aerobic glycolysis of gastric cancer cells. a-c AGS and HGC27 cells were exposed to 10 μg/mL Bupivacaine for 48 h. a and b Glucose, Oligomycin and 2-DG were sequentially injected. The ECAR was analyzed using the Seahorse XF 96 Extracellular Flux Analyzer to identify the glycolysis and glycolytic capacity. c Western blot assay was conducted to detect the expression of GLUT1 and LDHA in AGS and HGC27 cells treated with 10 μg/mL Bupivacaine for 48 h or not. **P < 0.01, ***P < 0.001