Fig. 5.

Circ_0000376 acts as a sponge of miR-145-5p in gastric cancer cells. a Circular RNA Interactome database was used to find the interacted-miRNAs of circ_0000376. The expression of these seven candidate miRNAs was measured in AGS cells transfected with si-NC or si-circ_0000376#2 via qRT-PCR. b The schematic represented the binding sites between circ_0000376 and miR-145-5p. The mutant binding sites with miR-145-5p in circ_0000376 were also shown as circ_0000376 mut. c The transfection efficiency of miR-145-5p in gastric cancer cells was evaluated by qRT-PCR. d and e Dual-luciferase reporter assay was conducted to analyze the direct binding between miR-145-5p and circ_0000376. AGS and HGC27 cells were co-transfected with miR-NC or miR-145-5p and circ_0000376 wt or circ_0000376 mut. The luciferase activities in these four different groups in AGS and HGC27 cells were detected. f MiR-145-5p level in si-NC or si-circ_0000376#2 transfected gastric cancer cells was measured by qRT-PCR. ***P < 0.001