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. 2020 Oct 30;8:151. doi: 10.1186/s40168-020-00930-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The Amazon River basin Microbial Non-Redundant Gene Catalogue (AMnrGC). a Distribution of the 106 metagenomes used in this work over the five sections of the Amazon River: Upstream (purple dots), Downstream (orange dots), Estuary (red dots), Plume (yellow dots) and coastal Ocean (white dots). b Taxonomic classification of the ~ 3.7 million genes in the AMnrGC. “Unassigned” genes were not assigned taxonomy, but they were functionally assigned, differently from “non-annotated” genes, which do not have any ortholog. Those genes displaying orthology to poorly characterized genes found in metagenomes were referred to as “Metagenomic”. c Rarefaction curves of non-redundant genes and PFAM families (internal plot). Note both point towards saturation. d NMDS comparing the Amazon river microbiome with other microbiomes based on information content [k-mer composition]. Amazon River (AMAZON), Amazon forest soil (FOREST), Canada watersheds (CANADA) and Mississippi River (MISSISSIPPI)