Fig. 2.

Scheme for construction of the plasmid containing the CO-GCSF gene. A copy of the CO-GCSF gene was amplified from pPICZαB-HSADIII-CO-GCSF construct [50] using G-CSF specific forward and reverse primers containing appropriate restriction sites. The restriction enzyme digested (Xho I and Xba I) vector and the amplified G-CSF fragment were isolated from the gel, purified and ligated to form the pPICZαB-CO-GCSF