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. 2020 Oct 29;19:199. doi: 10.1186/s12934-020-01460-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — a SDS-PAGE analysis of extracellular G-CSF produced in BMMY medium by the truncated Matα fused to the WT-GCSF gene. Equal volume (25 μl) of the culture filtrate was loaded after 72 h of cultivation in a 48-well plate. Representative image of Matα:Δ57–70 transformants is shown. Lanes 1 & 11: Mol wt markers; Lane 2: empty vector; Lanes 3, 4, 5, 6, 8, 9, 10: Cl #s 3 to 9; Lane 12: Matα:WT; Lanes 13, 14, 15, 17, 18, 19, 20: Cl #s 13 to 19; Lanes 7 & 16: Standard Filgrastim (2.0 μg & 2.5 μg respectively) b Average extracellular G-CSF (mg/L) production from 14/15 clones of each category at microplate level 72 h post-methanol induction