Fig. 5.

a SDS-PAGE analysis of the extracellular G-CSF produced in BMMY medium by the truncated Matα fused to the CO-GCSF. Equal volume (25 μl) of the culture filtrate was loaded after 72 h of cultivation in a 48-well plate. Representative image of Matα:Δ57–70 transformants is shown. Lanes 1 & 11: Mol wt markers; Lane 2: empty vector; Lane 3: Matα:WT fused to the CO-GCSF gene; Lanes 4 to 9: Cl #s 22, 23, 24, 25, 26, 27; Lanes 10 & 17: Standard Filgrastim (1.5 μg and 4.5 μg respectively); Lanes 12 to 19: Cl #s 28, 29, 30, 31, 32, 34, 35. b Average extracellular G-CSF (mg/L) production from 13 clones of each category at microplate level, 72 h post-methanol induction