Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 29;19:199. doi: 10.1186/s12934-020-01460-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — a SDS-PAGE analysis of extracellular G-CSF produced by transformants containing single deletions in the pro-secretory sequence fused to the CO-GCSF gene; Equal volume (10 μl) of the culture filtrate was loaded after 120 h of cultivation in a 500 ml Erlenmeyer flask. Lane 1: Molecular weight ladder; Lane 2; empty vector; Lane 3: Matα:WT fused to the CO-GCSF gene; Lanes 4 & 5: Matα:Δ57–70, Cl #s 25, 32; Lanes 6 & 7: Matα:Δ30–43, Cl# 12, 32; Lane 8: Filgrastim (1.5 μg); Lanes 9 & 10: Matα:Δ47–49, Cl #s 44, 45. b SDS-PAGE analysis of the G-CSF produced by transformants containing double deletions in the pro-secretory sequence fused to the CO-GCSF gene; Lane 11: Molecular weight ladder; Lane 12; Filgrastim (1.5 μg); Lane 13: Matα:WT fused to the CO-GCSF gene; Lanes 14 &15: Matα:Δ57–70, Cl# 25, 32; Lanes 16 &17: Matα:Δ57–70;47–49, Cl# 17,18; Lanes 18 & 19: Matα:Δ57–70;30–43, Cl# 23, 33. c Cell O.D, total extracellular protein and G-CSF titre at 120 h post-methanol induction. The units for cell O.D. are the same as shown on the Y-axis