Table 1.

Mutagenic primers used to carry out truncations in the α-MAT secretory signal

Primers	Primer Sequence
Matα:WT	GCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAG
Matα:Δ57–70	F.P: 5′-GCTGTTTTGCCATTTTCCGCCAGCATTGCTGCTAAAG-3’ R.P: 5′-CTTTAGCAGCAATGCTGGCGGAAAATGGCAAAACAGC-3’
Matα:WT	CTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCG
Matα:Δ30–43	F.P: 5′-CTACAACAGAAGATGAATTAGAAGGGGATTTCG-3’ R.P: 5′-CGAAATCCCCTTCTAATTCATCTTCTGTTGTAG-3’
Matα:WT	CGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCC
Matα:Δ47–49	F.P: 5′-CGGTTACTCAGATTTAGAAGGGGTTGCTGTTTTGCCATTTTCC-3’ R.P: 5′-GGAAAATGGCAAAACAGCAACCCCTTCTAAATCTGAGTAACCG-3’

The underlined sequences were removed in the different α-MAT deletion constructs. The corresponding WT α-MAT sequences are shown above the deletion