TABLE 2.
Microbial cell load (log cfu/g) of Limosilactobacillus reuteri DSM 20016, Bifidobacterium animalis subsp. lactis DSM 10140, and Lactiplantibacillus plantarum c16 and c19 immobilized for 15, 20, 25, and 30 min on apple pieces and stored at 4°C for 12 days.
| Microorganisms | Days |
Log cfu/g |
|||
| 15 | 20 | 25 | 30 | ||
| L. reuteri DSM 20016 | 0 | 7.61 ± 0.02 | 7.60 ± 0.08 | 7.60 ± 0.07 | 7.38 ± 0.02 |
| 2 | 7.70 ± 0.05 | 8.00 ± 0.06 | 8.08 ± 0.12 | 8.08 ± 0.12 | |
| 7 | 7.56 ± 0.50 | 7.26 ± 0.01 | 7.25 ± 0.07 | 7.64 ± 0.54 | |
| 12 | 7.72 ± 0.02 | 7.20 ± 0.01 | 7.98 ± 0.08 | 7.96 ± 0.24 | |
| ns* | ns | ns | ns | ||
| Bifidobacterium animalis subsp. lactis DSM 10140 | 0 | 8.07 ± 0.06 | 8.20 ± 0.14 | 8.40 ± 0.00 | 8.19 ± 0.10 |
| 2 | 7.96 ± 0.08 | 7.54 ± 0.61 | 7.61 ± 0.20 | 7.12 ± 0.16 | |
| 7 | 8.33 ± 0.03 | 8.30 ± 0.01 | 8.18 ± 0.23 | 8.14 ± 0.06 | |
| 12 | 8.33 ± 0.03 | 8.30 ± 0.01 | 8.00 ± 0.00 | 8.06 ± 0.08 | |
| Ns | ns | ns | ns | ||
| L. plantarum c16 | 0 | 7.98 ± 0.31 | 8.12 ± 0.25 | 8.00 ± 0.23 | 7.88 ± 0.17 |
| 2 | 8.36 ± 0.34 | 8.08 ± 0.16 | 8.33 ± 0.04 | 8.59 ± 0.01 | |
| 7 | 8.35 ± 0.06 | 8.32 ± 0.03 | 8.32 ± 0.03 | 8.27 ± 0.05 | |
| 12 | 8.16 ± 0.02 | 8.22 ± 0.03 | 8.26 ± 0.00 | 8.33 ± 0.04 | |
| Ns | ns | ns | ns | ||
| L. plantarum c16 | 0 | 8.45 ± 0.42 | 8.43 ± 0.15 | 8.42 ± 0.07 | 8.33 ± 0.09 |
| 2 | 8.12 ± 0.17 | 8.26 ± 0.18 | 8.34 ± 0.08 | 8.43 ± 0.38 | |
| 7 | 8.36 ± 0.08 | 8.35 ± 0.01 | 8.38 ± 0.01 | 8.43 ± 0.04 | |
| 12 | 8.10 ± 0.01 | 8.20 ± 0.04 | 8.33 ± 0.04 | 8.30 ± 0.01 | |
| Ns | ns | ns | ns | ||
Mean ± standard deviation. *ns, the data for each contact time were analyzed by one-way ANOVA and Tukey’s test and the differences were not significant (P > 0.05).