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. Author manuscript; available in PMC: 2021 Sep 9.
Published in final edited form as: Nature. 2020 Sep 9;585(7825):447–452. doi: 10.1038/s41586-020-2690-1

Extended Data Figure 7 (related to Figure 3). Non-centrosomal PCM foci nucleate microtubules and contribute to spindle assembly in RPE-1 cells.

Extended Data Figure 7 (related to Figure 3).

(A) Representative time-lapse images of mitosis in control RPE-1; PLK4AS; TP53−/− cells. n = 3, biological replicates. Scale bar, 5 μm.

(B) Representative time-lapse images of PCM foci formation during mitosis in acentrosomal RPE-1; PLK4AS; TP53−/− cells. n = 3, biological replicates. Scale bar, 5 μm. Arrows indicate PCM foci.

(C) Representative time-lapse images of microtubule nucleation from centrosomes in the mitotic spindle of control RPE-1; PLK4AS; TP53−/− cells. n = 3, biological replicates. Scale bars, 5 μm.

(D) Representative time-lapse images of microtubule nucleation from PCM foci incorporated into the mitotic spindle of acentrosomal RPE-1; PLK4AS; TP53−/− cells. n = 3, biological replicates. Scale bar, 5 μm.

(E) Representative time-lapse images of microtubule nucleation from a PCM focus prior to its incorporation into the mitotic spindle in acentrosomal RPE-1; PLK4AS; TP53-/−. n = 3, biological replicates. Scale bar, 1 μm.