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. Author manuscript; available in PMC: 2021 Sep 9.
Published in final edited form as: Nature. 2020 Sep 9;585(7825):447–452. doi: 10.1038/s41586-020-2690-1

Extended Data Figure 10 (related to Figure 4). TRIM37 overexpression delays centrosome maturation in G2/M phase.

Extended Data Figure 10 (related to Figure 4).

(A) Quantification of centrosomal α-tubulin intensity from time-lapse movies of dividing MCF-7 cells expressing either control or TRIM37-targeting shRNA. Quantitation of >20 cells. Mean ± s.e.m.

(B) Quantification of the distance between the two centrosomes at NEBD in MCF-7 cells. n = 3, biological replicates, each comprising >12 cells. P values, unpaired two-tailed t-test. Mean ± s.e.m.

(C) Quantification of centrosomal α-tubulin intensity from time-lapse movies of dividing RPE-1 tet-on TRIM37 cells. Quantitation of >20 cells. Mean ± s.e.m.

(D) Representative time-lapse images of centrosome maturation in MCF-7 cells. n = 3, biological replicates. Scale bars, 5 μm.

(E) Representative time-lapse images of centrosome maturation in RPE-1 tet-on TRIM37 cells. n = 3, biological replicates. Scale bars, 5 μm.