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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: Cell Chem Biol. 2019 Feb 7;26(4):535–547.e4. doi: 10.1016/j.chembiol.2019.01.002

Figure 2.

Figure 2.

Generation of the SGP and the Fmoc protected biantennary glycosylasparagine compound 1a. (a) A sialylated biantennary glycopeptide designated SGP (39) is abundantly available in chicken egg yolk and can be extracted by 40% acetone and purified by active charcoal/celite (2:1) column. Around 1.5 g SGP was harvested from 1.36 kg egg yolk powder. (b) Negative ion-mode MALDI-MS and positive ion-mode ESI-MS analyses of the purified SGP. (c) The SGP was converted to the target, Fmoc protected biantennary compound 1a, by mild acid hydrolysis, galactosidase and pronase digestion and Fmoc derivatization.