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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: Cell Chem Biol. 2019 Feb 7;26(4):535–547.e4. doi: 10.1016/j.chembiol.2019.01.002

Figure 3.

Figure 3.

Generation of the asparagine-linked N-glycans and reducing N-glycans. (a) and (b) Normal phase HPLC and MALDI-MS analyses of the substrate 1a and the product after 5% piperidine treatment. (c) and (d) Size exclusion chromatography and MALDI-MS analyses of the substrate 1 and the products after PNGase F or NaClO treatment. Dashes indicated the shift of the elution time of the product peak.