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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: Cell Chem Biol. 2019 Feb 7;26(4):535–547.e4. doi: 10.1016/j.chembiol.2019.01.002

Figure 5.

Figure 5.

Microarray analyses of recombinant human galectin-1 and -3. (a) and (b), The recombinant galectin-1 and -3 were tested at 5 and 50 μg/mL. Galectin-1 was detected by mouse anti-human galecin-1 followed by Alexa Fluor 488 labelled anti-mouse IgG (H+L). Galectin-3 was directly labelled with biotin and detected by cyanine 5-streptavidin. Results are shown as relative fluorescence units (RFUs) by averaging the background-subtracted fluorescence signals of 4 replicate spots, error bars represent the standard deviation among the 4 values. The RFUs and the standard deviation of 50 μg/mL were listed in Supplemental Table S2. The probes are grouped as indicated in the colored panels.