KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-human galectin-1 (clone # 933133) | R&D Systems | Cat#MAB1152 |
| Goat polyclonal anti-Human IgG (H+L), Alexa Fluor 488 | Thermo Fisher | Cat#A-11013 |
| Rabbit polyclonal anti-Mouse IgG (H+L), Alexa Fluor 488 | Thermo Fisher | Cat#A-11059 |
| Mouse monoclonal anti penta-His, Alexa Fluor 488 | Qiagen | Cat#35310 |
| Bacterial and Virus Strains | ||
| X-31 | John W. McCauley (Benton, D.J. et. al. 2015) | N/A |
| X-31 HAM | John W. McCauley (Benton, D.J. et. al. 2015) | N/A |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Egg yolk powder | Magic Flavors | N/A |
| Pronase | EMD Millipore | Cat#53702-250KU |
| Phosphatase | Sigma | Cat#P5931-200UN |
| β-galactosidase | Sigma | Cat#G5160 |
| Neuraminidase (Arthrobacter ureafaciens) | New England Biolabs | Cat#P0722L |
| UDP-Gal | Sigma; Chemily | Cat#U4500; Cat#SN02006 |
| UDP-GlcNAc | Chemily | Cat#SN02009 |
| GDP-Fucose | Chemily | Cat#SN02002 |
| CMP-sialic acid | Sigma; Chemily | Cat#233264; Cat#SN02001 |
| Fmoc N-hydroxysuccinimide ester | Sigma | Cat#46920-5G-F |
| 293 Freestyle expression medium | Thermo Fisher | Cat#12338018 |
| Polyethylenimene | Polysciences, Inc | Cat#23966 |
| Valproic acid | Sigma | Cat#P4543-100G |
| His-Pur Ni-NTA resin | Thermo Fisher | Cat#88221 |
| WGA | Vector Labs | Cat#B-1025 |
| DSA | Vector Labs | Cat#B-1185 |
| LCA | Vector Labs | Cat#B-1045 |
| PSA | Vector Labs | Cat#B-1055 |
| L-PHA | Vector Labs | Cat#B-1115 |
| E-PHA | Vector Labs | Cat#B-1125 |
| ECL | Vector Labs | Cat#B-1145 |
| RCA-I | Vector Labs | Cat#B-1085 |
| Con A | Vector Labs | Cat#B-1005 |
| SNA | Vector Labs | Cat#B-1305 |
| MAL-I | Vector Labs | Cat#B-1315 |
| GSL-II | Vector Labs | Cat#B-1215 |
| AAL | Vector Labs | Cat#B-1395 |
| GNA | Vector Labs | Cat#B-1245 |
| UEA-I | Vector Labs | Cat#B-1065 |
| Recombinant human Siglec-1/CD169 | R&D Systems | Cat#5197-SL |
| Recombinant human Siglec-2/CD22 Fc Chimera | R&D Systems | Cat#1968-SL |
| Recombinant human Siglec-3/CD33 Fc Chimera | R&D Systems | Cat#1137-SL |
| Recombinant human MAG/Siglec-4a Fc Chimera | R&D Systems | Cat#8940-MG |
| Recombinant human Siglec-5/CD105 Fc Chimera | R&D Systems | Cat#1072-SL |
| Recombinant human Siglec-6/CD327 Fc Chimera | R&D Systems | Cat#2859-SL |
| Recombinant human Siglec-7/Fc Chimera | R&D Systems | Cat#1138-SL |
| Recombinant human Siglec-8/Fc Chimera | R&D Systems | Cat#9045-SL |
| Recombinant human Siglec-9/Fc Chimera | R&D Systems | Cat#1139-SL |
| Recombinant human Siglec-10/Fc Chimera | R&D Systems | Cat#2130-SL |
| Recombinant human Siglec-11/Fc Chimera | R&D Systems | Cat#3258-SL |
| Recombinant Rat MAG/Siglec-4a Fc Chimera | R&D Systems | Cat#538-MG |
| Recombinant Human Galectin-1 | R&D Systems | Cat#1152-GA |
| Recombinant Human Galectin-3 | Laboratory stock (Noll, et al., 2016) | N/A |
| Streptavidin - Cyanine 5 | Invitrogen | Cat#434316 |
| Sulfo-NHS-LC-LC-Biotin | Thermo Scientific | Cat#21338 |
| Alexa Fluor-488 NHS | Thermo Scientific | Cat#A20000 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Human: Freestyle 293-F | Thermo Fisher | Cat#R79007 |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| Plasmid: B4GALT1-pGEn2-DEST | Kelley Moremen (Moremen et al, 2017) | HsCD00522296 |
| Plasmid: ST3GAL4-pGEn2-DEST | Kelley Moremen (Moremen et al, 2017) | HsCD00413174 |
| Plasmid: ST6GAL1-pGEn2-DEST | Kelley Moremen (Moremen et al, 2017) | HsCD00413052 |
| Plasmid: MGAT4A-pGEn2-DEST | Kelley Moremen (Moremen et al, 2017) | HsCD00413158 |
| Plasmid: MGAT5-pGEn2-DEST | Kelley Moremen (Moremen et al, 2017) | N/A |
| Plasmid: FUT8-pGEn2-DEST | Kelley Moremen (Moremen et al, 2017) | HsCD00413179 |
| Software and Algorithms | ||
| Other | ||
| Nexterion H NHS functionalized slides | Schott AG | Cat#1070936 |