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. Author manuscript; available in PMC: 2021 Sep 10.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Aug 6;530(1):278–284. doi: 10.1016/j.bbrc.2020.07.089

Figure 3: The IFN-γ / active STAT-1 signaling pathway is associated with neutrophil IL-10 generation.

Figure 3:

Peritoneal neutrophils of IL-10 GFP reporter mice were harvested and processed: A) 24 h after CLP while bone marrow derived, LPS-stimulated neutrophils were harvested: B) 24 h post stimulation. Active STAT-1 was determine as described in the materials and methods. To assess IFN-γ dependency of IL-10 production 100 μg anti-IFN-γ-Ab was pre-administered 24 hours prior to CLP surgeries. C) The proportion neutrophils generating IL-10 from mice treated with isotype or IFN-γ-neutralizing antibodies was determined as described. D) With IFN-γ blockade, IL-10 producing neutrophils are characterized as described in the material and methods. Data are expressed as means ± SD. *p < 0.05.