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. Author manuscript; available in PMC: 2021 Sep 10.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Aug 6;530(1):278–284. doi: 10.1016/j.bbrc.2020.07.089

Figure 4: CD4 T cells are the most prevalent IFN-γ producers during acute sepsis.

Figure 4:

Twenty-four hours prior to the CLP surgeries, 250 μg of Brefeldin A were injected into wild type C57BL/6J mice. Peritoneal cells were harvested 3 hours after CLP with intracellular labeling for IFN-γ expression conducted as described in material and methods. As described in the material and methods, A) Leukocyte numbers were determined. B) Leukocyte proportions analyzed, C) CD4 phenotype and D) CD8 phenotype was determined for IFN-γ expression. Data are expressed as means ± SD. *p < 0.05.