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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: J Vis Exp. 2019 Nov 7;(153):10.3791/60472. doi: 10.3791/60472

Figure 1: Experimental setup.

Figure 1:

(A) Diagram of the isolated heart perfusion system; arrows denote the direction of flow. (B) A cannulated heart is shown with electrode placement. RA = right atria, RV = right ventricle, LV = left ventricle, ECG = lead II electrocardiogram. (C) The imaging platform in close proximity to the heart tissue. (D) Emission of each complementary probe (voltage, calcium) is separated by wavelength using an image splitting device with appropriate emission filters and dichroic mirror.