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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: J Vis Exp. 2019 Nov 7;(153):10.3791/60472. doi: 10.3791/60472

Figure 5: Optical data from Langendorff-perfused pig hearts.

Figure 5:

Unprocessed, spatially filtered (A) transmembrane voltage and (B) intracellular calcium fluorescence signals from the right and left ventricles during electrical pacing at the apex. Unfiltered, spatially averaged signals depict optical action potentials and calcium transients from regions of interest (signal units are ΔF/F). (C) An overlay of normalized transients illustrates action potential-calcium transient coupling time (low-pass filtered at 75 Hz). (D) Processing signals across the epicardial surface to generate isochronal maps of temporal parameters, including activation time (tact) and 80% repolarization time. (E) Electrical and calcium transient restitution curves generated at multiple frequencies (left) with statistical analysis (right) to illustrate longer repolarization time at slower pacing cycle lengths. Scale bars = mean ± SEM.