. 2020 Mar 13;99(5):2608–2615. doi: 10.1016/j.psj.2019.12.054

Table 7.

Effect of dietary 25-OH-D₃ on serum hormone concentrations of laying hens under high or low stocking density¹.

Item	25-OH-D₃	CORT	LPS	CA	OC	PTH	CT
Item	ng/mL	ng/L	ng/L	ng/L	ng/L	ng/L	ng/L
Density 25-OH-D₃
Low−	7.65 ± 0.34^b	455.13 ± 12.57^a	195.09 ± 0.47^a,b	311.91 ± 16.54^c	498.94 ± 20.56^b	217.23 ± 10.56^a	69.87 ± 3.21^b
Low+	8.15 ± 0.51^b	367.00 ± 17.86^b	181.71 ± 0.47^b	354.80 ± 19.65^a,b	469.52 ± 18.65^b	200.66 ± 12.89^a,b	77.93 ± 2.99^a,b
High−	7.86 ± 0.61^b	459.78 ± 9.67^a	212.10 ± 0.47^a	328.87 ± 12.56^b,c	579.30 ± 26.89^a	160.36 ± 7.48^c	68.78 ± 4.98^b
High+	9.20 ± 0.47^a	427.93 ± 19.21^a	195.19 ± 0.47^a,b	373.86 ± 21.44^a	481.80 ± 16.98^b	182.26 ± 10.55^b,c	83.21 ± 5.07^a
P-value	<0.01	<0.01	0.05	0.05	<0.01	<0.01	0.05
Main effect P-value
Density	0.05	0.05	0.05	0.19	0.05	<0.01	0.53
25-OH-D₃	<0.01	<0.01	0.05	<0.01	<0.01	0.74	<0.01
Density*25-OH-D₃	0.09	0.06	0.81	0.94	0.07	0.05	0.34

^a-bMeans with different superscripts within a column differ significantly (P < 0.05).

Abbreviations: 25-OH-D3, 25-hydroxycholecalciferol; CA, carbonic anhydrase; CORT, corticosteron; CT, calcitonine; LPS, lipopolysaccharide; OC, osteocalcin; PTH, parathyroid hormone.

Each mean represents 10 replicates per treatment, with 20 layer per replicate.