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. 2020 Oct 14;11:564288. doi: 10.3389/fimmu.2020.564288

Figure 7.

Figure 7

Analyses of cellular proinflammatory signaling pathways and apoptosis of hypoxic HK-2 cells. (A) Western blotting of TLR4 and NFκB showed that JPI-289 treatment reduced the expressions of TLR4 and NFκB compared with the hypoxia control group. Data are from six independent experiments. *P < 0.05, compared with the hypoxia control group. Statistical analysis was performed with the Mann-Whitney U-test. CD0, normoxia control group on day 0; CD1, normoxia control group on day 1; CD2, normoxia control group on day 2; HD0, the hypoxia group on day 0 in normoxia; HD1, the hypoxia group on day 1 in normoxia; HD2, the hypoxia group on day 2 in normoxia. The concentration of JPI-289 (ng/ml) is expressed in the parentheses. (B) Enzyme-linked immunosorbent assay of Bax and Bcl-2 showed that JPI-289 treatment reduced Bax/Bcl-2 ratios. Data are from six independent experiments. *P < 0.05, compared with the hypoxia control group. Statistical analysis was performed with the Mann-Whitney U test.