Table 1.

The leukocyte populations in the postischemic kidney on day 3 after IRI.

Leukocytes’ types (% in parent gate)	Control	JPI-289 50 mg/kg	JPI-289 100 mg/kg
Of size and granularity-based gate
Macrophages, %  NK T cells, %  NK cells, %	39.0 ± 2.6 2.5 ± 0.3 16.7 ± 2.5	30.8 ± 2.1* 1.9 ± 0.2 10.4 ± 1.0*	33.9 ± 2.2* 2.5 ± 0.2 11.4 ± 1.4*
Of size and granularity-based gate
Neutrophils, %	4.9 ± 0.8	2.7 ± 0.3	3.2 ± 0.3
Among total lymphocytes
Total T cells, %	25.1 ± 1.7	23.7 ± 1.1	22.3 ± 2.4
Total B cells, %	37.1 ± 2.5	42.1 ± 4.2	42.3 ± 1.9
Among total T cells
CD4 T cells, %	67.9 ± 2.1	63.6 ± 1.6	65.8 ± 2.0
CD8 T cells, %	12.7 ± 1.6	15.1 ± 1.1	13.0 ± 1.7
Among total CD4 T cells
Activated CD4 T cells, %	3.2 ± 0.3	3.2 ± 0.4	3.7 ± 0.3
Effector memory CD4 T cells, %	39.5 ± 2.9	39.6 ± 3.6	42.8 ± 4.9
Regulatory T cells, %	0.7 ± 0.1	0.5 ± 0.1	0.8 ± 0.2
Among total CD8 T cells
Activated CD8 T cells, %	2.3 ± 0.6	2.5 ± 0.4	2.1 ± 0.6
Effector memory CD8 T cells, %	14.9 ± 2.6	15.2 ± 3.2	20.3 ± 5.2
Among total B cells
Activated B cells, %	1.1 ± 0.2	1.1 ± 0.2	1.1 ± 0.1
Mature B cells, %	93.3 ± 0.7	93.5 ± 1.2	94.6 ± 0.5
Memory B cells, %	5.1 ± 0.2	5.5 ± 0.7	6.1 ± 0.4
Plasma cells among KMNCs	0.3 ± 0.1	0.2 ± 0.1	0.3 ± 0.1

Data (mean ± standard error of mean) are from two independent experiments. *P < 0.05, compared with the control group (n = 6–8 mice in each group).

Detailed gating strategies are provided in Supplemental Materials .

IRI, ischemia-reperfusion injury; KMNCs, kidney mononuclear cells.