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. 2020 Mar 18;99(5):2595–2607. doi: 10.1016/j.psj.2019.12.051

Table 1.

The ingredients and nutrient composition of positive control and negative control diets fed to laying hens from 25–37 wk of age.

25–28 wk
29–37 wk
PC1 NC2 PC1 NC2
Ingredients (%)
 Corn 58.52 61.03 59.63 62.04
 Soybean meal 18.96 18.96 16.47 16.47
 Canola meal 10.00 10.00 12.00 12.00
 Canola oil 1.44 1.44 0.96 0.96
 Calcium carbonate 8.41 7.23 8.67 7.35
 Dicalcium phosphate 1.76 0.50 1.37 0.33
 Salt 0.32 0.24 0.29 0.24
 DL-Methionine 0.10 0.10 0.10 0.10
 Vitamin-mineral premix3 0.50 0.50 0.50 0.50
 Phytase4 (g/kg) Variable5 Variable5 Variable5 Variable5
Calculated nutrient composition (%)
 ME (kcal/kg) 2,800 2,885 2,775 2,857
 Crude protein 18.00 18.20 17.60 17.80
 Calcium 3.70 3.00 3.73 3.02
 Available phosphorus 0.45 0.22 0.38 0.19
 Phytate phosphorus 0.25 0.26 0.26 0.27
 Total phosphorus 0.70 0.48 0.64 0.46
 Sodium 0.16 0.13 0.15 0.13
1

PC = positive control diet; PC diet was mixed as a single batch and subdivided into the PC and PC + phytase treatments.

2

NC = negative control diet; NC diet was mixed as a single batch and subdivided into the NC and various NC + phytase treatments.

3

Vitamin–mineral premix (units per kilogram of feed): vitamin A, 12,500 IU; vitamin D3, 3,125 IU; vitamin E, 40 IU; vitamin K (menadione), 2.5 mg; riboflavin, 7.5 mg; D-pantothenic acid, 12.5 mg; vitamin B12, 0.01875 mg; pyridoxine, 5 mg; thiamine, 2.55 mg; folic acid, 0.625 mg; niacin, 37.5 mg; biotin, 0.15 mg; iodine, 1.65 mg; copper, 15 mg; iron, 80 mg; selenium, 0.3 mg; manganese, 88 mg; zinc, 100 mg.

4

Quantum Blue phytase (5,000 FTU/g of premix; AB Vista, Marlborough, UK).

5

Quantum Blue phytase was added on top of the NC diet at 0.03 (150 FTU/kg), 0.06 (300 FTU/kg), 0.12 g/kg (600 FTU/kg) or 0.24 (1,200 FTU/kg) g/kg for the NC phytase-containing diets, or on top of the PC diet at 0.24 g/kg (1,200 FTU/kg) for the PC phytase-containing diet.