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. 2020 Mar 18;99(5):2595–2607. doi: 10.1016/j.psj.2019.12.051

Table 2.

Analyzed calcium, total phosphorus and phytase activity levels of experimental layer diets1,2.

Diet3
PC NC NC + 150 NC + 300 NC + 600 NC + 1,200 PC + 1,200
Calcium (% of the diet, as-fed basis)
 25–28 wk 4.64 (0.06) 3.22 (0.10) 2.74 (0.15) 2.52 (0.11) 3.64 (0.07) 3.16 (0.07) 4.13 (0.17)
 29–34 wk 4.22 (0.08) 4.10 (0.04) 3.90 (0.09) 4.32 (0.07) 3.47 (0.03) 3.30 (0.09) 4.37 (0.13)
 35–37 wk4 4.35 (0.12) 2.38 (0.11) 3.18 (0.12) 2.94 (0.13) 2.98 (0.08) 2.90 (0.12) 4.09 (0.11)
Total phosphorus (% of the diet, as-fed basis)
 25–28 wk 0.63 (0.03) 0.48 (0.01) 0.46 (0.01) 0.45 (0.03) 0.49 (0.01) 0.47 (0.03) 0.60 (0.01)
 29–34 wk 0.58 (0.01) 0.45 (0.03) 0.46 (0.01) 0.44 (0.01) 0.41 (0.03) 0.47 (0.01) 0.61 (0.03)
 35–37 wk4 0.59 (0.04) 0.31 (0.01) 0.45 (0.03) 0.38 (0.01) 0.41 (0.04) 0.43 (0.02) 0.50 (0.03)
Phytase activity5 (FTU/kg)
 25–28 wk <50 <50 267 (13) 421 (50) 875 (0) 2,000 (0) 1,820 (91)
 29–37 wk <50 <50 254 (33) 442 (35) 743 (0) 1,540 (231) 1,570 (173)
1

Feed provided in mash form.

2

Means of 2 replicate feed samples, standard deviations are given in parentheses. Phytase activity in the PC and NC diets were lower than the detection level, therefore means and standard deviations were not calculated.

3

PC, a positive control diet, nutritionally complete diet containing 0.45% aP, 3.70% Ca, and 0.16% Na (25–28 wk) and 0.38% aP, 3.73% Ca, and 0.15% Na (29–37 wk); NC, a negative control diet, similar to the PC diet but having 0.22% aP, 3.00% Ca, and 0.13% Na (25–28 wk) and 0.19% aP, 3.02% Ca, and 0.13% Na (29–37 wk); NC + 300, NC + 600, and NC + 1,200, the NC diet supplemented with 300, 600, or 1,200 FTU Quantum Blue phytase (AB Vista, Marlborough, UK)/kg, respectively; and PC + 1,200, the PC diet supplemented with 1,200 FTU Quantum Blue phytase/kg.

4

Value for each diet was used to calculate AID of P and Ca for each respective treatment.

5

Analyzed by Enzyme Services Consultancy (Ystrad Mynach, UK) using Quantiplate ELISA Kits for Quantum Blue (Envirologix method AP181 with some modifications; designated Enzyme Services Consultancy Standard Analytical Method SAM099).