Table 1.
Sequences of primers used for quantitative real-time PCR.
| Gene symbol | Group | Gene title | Primer sequence (5ʹ-3ʹ) | Ta °C |
Amplicon size (bp) | Reference | Function |
|---|---|---|---|---|---|---|---|
| CLDN1 | Tight junction protein | Claudin 1 | F-CTTCATCATTGCAGGTCTGTCAG R-AAATCTGGTGTTAACGGGTGTG |
60 | 103 | This study | Maintenance of intestinal barrier function |
| CLDN5 | Tight junction protein | Claudin 5 | F-GCAGGTCGCCAGAGATACAG R-CCACGAAGCCTCTCATAGCC |
61 | 162 | This study | Maintenance of intestinal barrier function |
| JAM2D | Tight junction protein | Junctional adhesion 2 | F-AGACAGGAACAGGCAGTGCTAG R-ATCCAATCCCATTTGAGGCTAC |
60 | 135 | This study | Maintenance of intestinal barrier function |
| TJP1 | Tight junction protein | Tight junction protein | F-GGATGTTTATTTGGGCGGC R-GTCACCGTGTGTTGTTCCCAT |
60 | 187 | This study | Maintenance of intestinal barrier function |
| OCLD | Tight junction protein | occludin | F- ACGGCAGCACCTACCTCAA R- GGGCGAAGAAGCAGATGAG |
60 | 123 | (Du, et al., 2016a) | Maintenance of intestinal barrier function |
| APN | Amino acid transporter | Aminopeptidase N | F-AATACGCGCTCGAGAAAACC R-AGCGGGTACGCCGTGTT |
60 | 70 | (Gilbert, et al., 2007) | Amino acid transporter |
| ASCT | Amino acid transporter | Alanine, serine, cysteine, and threonine transporter | F-TTGGCCGGGAAGGAGAAG R-AGACCATAGTTGCCTCATTGAATG |
60 | 63 | (Paris and Wong, 2013) | Amino acid transporter |
| NaPi-IIb | Phosphorus transporter | Na-dependent Pi cotransporters, type IIb | F- CGGTCCGTTCACTCTGTTGC R- GAAGCCACGTTGCCTTTGTG |
60 | 166 | This study | Mediation of intestinal phosphate uptake |
| VDR | Vitamin D transporter | Vitamin D receptor | F- ACGCAGACATGGACACCGT R- GGACAGGTGAACATCGCTTTC |
60 | 193 | This study | Provides instructions for making vitamin D receptor |
| CALB1 | Calcium transporter | Calbindin 1 | F- GGCAGGCTTGGACTTAACACC R- GTCGGCAACACCTGAGCAAG |
60 | 105 | This study | Transport protein of calcium |
| ATP1A1 | Calcium transporter | ATPase Na+/K+ transporting subunit alpha 1 | F- GTCAACCCGAGGGATGCTAA R- ACTGCTACAATGGCACCCTG |
60 | 179 | (Kheravii, et al., 2018) | Provides instructions for making Na+/K+ ATPase |
| ATP5A1W | Calcium transporter | ATP synthase subunit alpha | F- GGCAATGAAACAGGTGGCAG R- GGGCTCCAGCTTGTCTAAGTGA |
60 | 232 | This study | Synthesis of ATP |
| ATP13A4 | Calcium transporter | ATPase type 13A4 | F-CCAAAGCTCCTGCTAAATGC R-ATGCCTCCTGCTCTGACAGT |
61 | (Lee and Kim, 2018) | Synthesis of ATP | |
| ATP2A1 | Calcium transporter | ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 | F-CGCTGTCAATCAGGACAAGA R-GTCGTTAAAGTGGCCGATGT |
61 | 250 | (Lee and Kim, 2018) | Catalyzes the hydrolysis of ATP and translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. |
| ATP2B1 | Calcium transporter | ATPase, Ca++ transporting, plasma membrane 1 | F-CTGGGCATGGGAACACTACT R-CACGACGTAATTCTCGCTCA |
60 | 169 | (Lee and Kim, 2018) | Catalyzes the hydrolysis of ATP and maintenance of intracellular calcium homeostasis |
| CASR | Calcium transporter | Calcium-sensing receptor | F-CTGCGTGATTTGGCTCTACA R-GGCAAAGAAGAAGCAGATGG |
60 | 160 | (Lee and Kim, 2018) | Regulator of parathyroid hormone synthesis and secretion and systemic calcium homeostasis |
| SLC8A1 | Calcium transporter | Solute carrier family 8 (sodium/calcium exchanger), member 1 | F-TCGTACCCCCGACAGACTAC R-GCCACCTTACTGGCAAATGT |
60 | 196 | (Lee and Kim, 2018) | Mediates the exchange of one Ca2+ against 3 to 4 Na+ ions across the cell membrane |
| SLC8B1 | Calcium transporter | (sodium/lithium/calcium exchanger), member B1 | F-CTTCGAGCTGAGCAACACTG R-CCCACACCCACCAGAATATC |
60 | 169 | (Lee and Kim, 2018) | Mediates mitochondrial calcium extrusion and mitochondrial calcium homeostasis |
| CACNA1A | Calcium channel | Calcium channel, voltage-dependent, P/Q type alpha 1A subunit | F-GCAGCGGGTCTATAAGCAGT R-GCGATGATGGTGGCTAAAAT |
60 | 198 | (Lee and Kim, 2018) | Provides instructions for making calcium channels |
| CACNB1 | Calcium channel | Calcium channel, voltage-dependent, beta 1 subunit | F-GGCAGCATATGGTGTGTGAC R-GGACAAAATCCTGGTGCTGT |
61 | 206 | (Lee and Kim, 2018) | Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit |
| CACNG1 | Calcium channel | calcium channel, voltage-dependent, gamma subunit 1 | F-CAGCTACTCCTGGTCCTTCG R-GGAGATCCCGACATCTGAAA |
60 | 216 | (Lee and Kim, 2018) | Plays a role in excitation-contraction coupling |
| TPCN1 | Calcium channel | Two pore segment channel 1 | F-GACGGCCTGTCTCTGAGTTC R-ATCCCACTGCCTGATTTACG |
60 | 186 | (Lee and Kim, 2018) | Very little is known about the physiological functions but believe to form functional Ca2+-permeable channel |
| TPCN2 | Calcium channel | Two pore segment channel 2 | F-AGGTGCTGTGGTTCCTATGG R-GCCCACGGACTTGTGTATCT |
60 | 210 | (Lee and Kim, 2018) | Very little is known about the physiological functions but believe to form functional Ca2+-permeable channel |
| TPCN3 | Calcium channel | Two-pore calcium channel 3 | F-TGGGAATGGGAGTTCAAGAG R-CTGCCTCAAACATACGCTGA |
60 | 183 | (Lee and Kim, 2018) | Very little is known about the physiological functions but believe to form functional Ca2+-permeable channel |
| MUC2 | Inflammatory genes | Mucin 2 | F- CCCTGGAAGTAGAGGTGACTG R- TGACAAGCCATTGAAGGACA |
143 | 60 | (Fan, et al., 2015) | The physical and biological barrier protecting mucousepithelia |
| MUC-5AC | Inflammatory genes | Mucin 5 | F- AAGACGGCATTTATTTCTCCAC R- TCATTACCAACAAGCCAGTGA |
244 | 60 | (Fan, et al., 2015) | The physical and biological barrier protecting mucous epithelia |
| HPRT1 | Housekeeping genes | Hypoxanthine guanine phosphoribosyl transferase 1 | F- ACTGGCTGCTTCTTGTG R- GGTTGGGTTGTGCTGTT |
62 | 245 | (Yang, et al., 2013) | Provides instructions for making hypoxanthine phosphoribosyl transferase 1 |
| GAPDH | Housekeeping genes | Glyceraldehyde-3-phosphate dehydrogenase | F: GAAGCTTACTGGAATGGCTTTCC R: CGGCAGGTCAGGTCAACAA |
61 | 66 | (Kuchipudi, et al., 2012) | Energy metabolism and the synthesis of ATP and pyruvate |