Table 5.
Effect of meat and bone meal, phytase, antibiotics, and necrotic enteritis on leucocytes, day 16 post-hatch.
| Effects | MBM | Phy | AB | WBC | Heterophils (106/mL) 13–17 |
Lymphocytes (106/mL) 110–30 |
Monocytes (106/mL) 10–5 |
Eosonophils (106/mL) 10–0.5 |
Basophils (106/mL) 20.3–2.5 |
|---|---|---|---|---|---|---|---|---|---|
| 2-way interactions | |||||||||
| MBM1AB | |||||||||
| − | − | 63.96 | 10.56 | 29.03a | 11.54 | 0.67 | 12.16 | ||
| − | + | 67.51 | 28.99 | 17.95b | 14.02 | 2.77 | 14.73 | ||
| + | − | 66.00 | 25.55 | 22.25a,b | 12.47 | 2.01 | 12.30 | ||
| + | + | 70.46 | 34.99 | 25.35a,b | 9.95 | 3.28 | 11.63 | ||
| Main effects | |||||||||
| Phy | 500 | 61.32 | 22.03 | 24.53 | 10.80 | 2.02 | 10.42 | ||
| 1,500 | 72.65 | 28.02 | 23.25 | 13.19 | 2.34 | 14.99 | |||
| P > f | |||||||||
| MBM | 0.697 | 0.419 | 0.726 | 0.630 | 0.460 | 0.643 | |||
| Phy | 0.083 | 0.643 | 0.580 | 0.465 | 0.795 | 0.157 | |||
| AB | 0.532 | 0.284 | 0.137 | 0.995 | 0.180 | 0.767 | |||
| MBM × Phy | 0.439 | 0.429 | 0.585 | 0.993 | 0.228 | 0.448 | |||
| MBM × AB | 0.943 | 0.728 | 0.002 | 0.443 | 0.736 | 0.613 | |||
| Phy × AB | 0.302 | 0.975 | 0.713 | 0.937 | 0.689 | 0.522 | |||
| MBM × Phy × AB | 0.196 | 0.252 | 0.606 | 0.566 | 0.242 | 0.140 |
a,b,cMeans in the same column within the main effect, 2-way interaction or treatment means with different superscripts are different (P < 0.05).
2- or 3-way interaction separated by Tukey.
Abbreviations: AB, salinomycin 60 ppm in S, G, F; Zn bacitracin 100 ppm in S, G and 50 ppm in F; MBM, meat and bone meal; Phy, phytase (Quantum Blue 5G); WBC, white blood cells.