. 2020 Mar 11;99(5):2581–2594. doi: 10.1016/j.psj.2019.12.049

Table 7.

Effect of meat and bone meal, phytase, antibiotics, and necrotic enteritis on jejunal gene expression, day 16 post-hatch.

Effects	MBM	Phy	AB	CALB1	MUC2	MUC-5AC	ATP1A1	ATP13A4	CACNA1	CACNB1	TPCN3
Treatment means
1	−	500	−	1.111	1.088	0.989	1.065	1.146	1.221^b,c	1.202	1.098
2	−	1,500	−	1.295	1.564	1.226	1.339	1.227	1.964^a	1.130	0.947
3	−	500	+	2.329	1.113	1.467	1.353	0.849	1.557^a,b	0.919	1.068
4	−	1,500	+	1.938	1.354	1.450	1.275	1.283	1.549^a,b	0.961	1.050
5	+	500	−	0.780	1.120	0.976	1.162	1.241	1.347^b	1.422	1.409
6	+	1,500	−	1.071	0.901	0.969	0.809	0.924	0.885^c	0.974	0.968
7	+	500	+	1.171	0.961	1.017	1.079	1.025	0.762^c,d	0.965	0.876
8	+	1,500	+	1.41	1.112	1.155	1.090	1.007	1.502^b	1.280	1.083
2-way interactions
MBM*Phy	−	500		1.720	1.101	1.228	1.209	0.998^b	1.389	1.060	1.083
	−	1,500		1.617	1.459	1.338	1.307	1.255^a	1.757	1.045	0.998
	+	500		0.975	1.040	0.997	1.121	1.133^a	1.055	1.194	1.142
	+	1,500		1.242	1.007	1.062	0.949	0.966^b	1.757	1.127	1.026
Phy*AB
		500	−	0.946	1.104	0.983	1.114	1.193	1.284	1.312^a	1.253^a
		500	+	1.750	1.037	1.242	1.216	0.937	1.159	0.942^b	0.972^b
		1,500	−	1.183	1.233	1.098	1.074	1.075	1.425	1.052^b	0.957^b
		1,500	+	1.675	1.233	1.302	1.182	1.145	1.526	1.120^a,b	1.067^a,b
Main effects
MBM	−			1.668^a	1.280^a	1.283	1.258	1.126	1.573	1.053	1.041
	+			1.109^b	1.024^b	1.029	1.035	1.049	1.124	1.160	1.084
AB			−	1.064^b	1.168	1.040	1.094	1.134	1.355	1.182	1.105
			+	1.713^a	1.135	1.272	1.199	1.041	1.343	1.031	1.019
P > f
MBM				0.044	0.044	0.070	0.073	0.495	0.112	0.405	0.703
Phy				0.764	0.194	0.523	0.764	0.689	0.364	0.751	0.378
AB				0.021	0.788	0.096	0.390	0.408	0.965	0.244	0.449
MBM × Phy				0.497	0.118	0.868	0.272	0.065	0.682	0.841	0.886
MBM × AB				0.302	0.632	0.389	0.959	0.809	0.920	0.559	0.091
Phy × AB				0.566	0.788	0.842	0.979	0.152	0.684	0.093	0.283
MBM × Phy × AB				0.628	0.225	0.467	0.147	0.904	0.085	0.212	0.258

^a,b,c Means in the same column within the main effect, 2-way interaction or treatment means with different superscripts are different (P < 0.05).

2-way interaction separated by least square difference; 3-way interaction by Tukey.

Abbreviations: AB, salinomycin 60 ppm in S, G, F; Zn bacitracin 100 ppm in S, G and 50 ppm in F; ATP13A4, ATPase type 13A4; ATP1A1, ATPase Na+/K+ transporting subunit alpha 1; CALB1, calbindin 1; CACNA1, calcium channel; CACNB1, calcium channel, voltage-dependent, beta 1 subunit; MBM, meat and bone meal; MUC2, mucin 2; MUC-5AC, mucin 5; Phy, phytase (Quantum Blue 5G); TPCN3, two-pore calcium channel 3.