Figure 1.

Identification of cells expressing mucin2 mRNA or mucin glycoprotein. Serial sections were cut from formalin-fixed paraffin-embedded samples of the jejunum of 2-day-old chicks. One section (A) was stained with alcian blue and periodic acid Schiff to detect acidic and neutral mucin glycoproteins, whereas the other section (B) was processed for in situ hybridization using the RNAscope 2.5 HD kit (Brown) and a mucin2 (Muc2) probe. Tissues were counterstained with hematoxylin. Cells expressing acidic mucin glycoprotein stained blue (A), whereas cells expressing Muc2 mRNA stained brown (B). Images were captured at 100X magnification (n = 2).