Table 2.
Nucleotide sequences of specific primers.
| Target genes | GenBank accession | Primer sequences (5′ to 3′) | PCR products (bp) |
|---|---|---|---|
| SREBP1 | AY029224 | F: CTACCGCTCATCCATCAACG R: CTGCTTCAGCTTCTGGTTGC |
145 |
| SREBP2 | XM_416222 | F: CCCAGAACAGCAAGCAAGG R: GCGAGGACAGGAAAGAGAGTG |
108 |
| HMGCR | NM_204485.1 | F: TTGGATAGAGGGAAGAGGGAAG R: CCATAGCAGAACCCACCAGA |
137 |
| CYP7A1 | AB109636.1 | F: CATTCTGTTGCCAGGTGATGTT R: GCTCTCTCTGTTTCCCGCTTT |
106 |
| CYP27A1 | XM422056.4 | F: AGGACTTTCGTCTGGCTCT R: CTCCGCATCGGGTATTT |
185 |
| ABCA1 | NM_204145.2 | F: TCCTCTGGCTTAGACTTGA R: CTCGTAGTTGTATTCGGTAA |
130 |
| APOA1 | NM_205525.4 | F: GTGACCCTCGCTGTGCTCTT R: CACTCAGCGTGTCCAGGTTGT |
217 |
| ACAT1 | NM_001277779.1 | F: TAATGGTTGCTGGTGG R: GCTTTGCTCTTGGTGT |
232 |
| LDLR | NM_204452.1 | F: CCACCATTTGGCAGAGGAA R: ACCGCAGTCAGACCAGAAGAG |
86 |
| RN18S | DQ018752 | F: ATAACGAACGAGACTCTGGCA R: CGGACATCTAAGGGCATCACA |
136 |
| MeDIP | |||
| SREBP2 promoter | F: GCTCTTGGGAGTTGTC R: AGGTTGTTCCAGTTGG |
224 | |
| CYP7A1 promoter | F: GAAAGATGTCAGAGCAG R: ACTATGGCAGAAGAGG |
160 | |
| ACAT1 promoter | F: TGTCAGTCCCCGCTCT R: CCTGGTAGGCATTTCTT |
190 |