Relative abundances of Campylobacter, Enterobacteriaceae, and Staphylococcus in the ceca and lung microbiomes of CF hens. Bars represent the mean ± SEM relative abundance of sequences from 20 hens in a cage free (CF) environment at early (white), peak (light gray), and late (dark gray) lay. Data for the ceca is displayed on the left side and lung on the right side. The relative abundances are shown for (A) Campylobacter in the ceca and (B) lung, (C) Enterobacteriaceae in the ceca and (D) lung, (E) Staphylococcus in the ceca and (F) lung. Statistical significance was determined using a one-way ANOVA with Tukey for multiple testing correction. ∗∗, P < 0.01. ∗∗∗, P < 0.001.