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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: FASEB J. 2020 Mar 24;34(5):6613–6627. doi: 10.1096/fj.201903226RR

FIGURE 4.

FIGURE 4

Activation of energy-sensing signaling pathways and impaired fasting-to-feeding metabolic transition in Bmal1-deficient muscle. A-C, Skeletal muscle total NAD++NADH (A), NADH levels (B) and NAD+/NADH ratio (C) in TA muscle of mBMCtr and mBMKO mice in response to feeding and fasting (n = 6–7/group). D and E, Immunoblot analysis of PGC-1a protein expression (D), and AMPK phosphorylation (E) under fasting or refeeding conditions in mBMCtr and mBMKO mice (n = 3/group). F, Immunoblot analysis of Glut4 and PDK4 protein regulations, and (G, H) RT-qPCR analysis of genes involved in fat oxidation (G) and glucose metabolism (H) in response to fasting and refeeding in mBMCtr (n = 6) and mBMKO (n = 5) mice. *,**P < .05 or .01 mBMKO vs mBMCtr; $,$ $P < .05 & .01 fasted vs refed by two-way ANOVA