Fig. 2.

Synovial explant‐conditioned media (ECM) induces metabolic alterations in dendritic cells (DC). (a) The relative expression of hexokinase 2 (HK2), pyruvate dehydrogenase kinase (PDK)1, PDK2, glycogen synthase kinase 3 alpha (GSK3a), hexose‐6‐phosphate dehydrogenase (H6PD) and 6‐phosphofructo‐2‐kinase/fructose‐2,6‐biphosphatase 3 (PFKFB3) in monocyte‐derived DC (MoDC) left untreated (basal) or treated with 10% ECM for 6 h (n = 6). (b) Dot‐plots and representative histograms of 2NBDG (glucose uptake) (n = 7) and CD98 mean fluorescence intensity (MFI) (n = 4) on MoDC treated with 10% ECM for 6 h. Paired t‐test was used. *P < 0·05, **P < 0·01, ***P < 0·001 significantly different from basal.