Fig. 1.

Reduced H19 imprinting control region (ICR) methylation in saliva derived from Sjögren’s syndrome (SS) patients compared to sicca controls. (a) Schematic representation of the H19 ICR genomic region analyzed by methylation‐sensitive restriction digestion and polymerase chain reaction (PCR). Arrows labelled F and R indicate forward and reverse primers used in the polymerase chain reaction. (b) Analysis using methylation‐sensitive restriction endonuclease HpaII followed by quantitative real‐time PCR. Values were normalized to MspI digestion control reactions. (c) No difference was observed in saliva Sjögren’s syndrome antigen B (SSB P1) methylation by the same method. (d) No difference was observed in H19 ICR methylation between male and female donors. (e) Significant negative correlation is observed between patient C4 levels and H19 ICR saliva methylation.