Figure 5. Diversity and taxonomic profiles of the metagenomic sequencing.

(A) Boxplot showing the Shannon diversity index for the taxonomic profiling for the three groups. Significance was tested using a One-way ANOVA with Student-Neuman Keul’s post-hoc analysis. ^∗∗∗p-value <0.001, ^∗∗∗∗p-value <0.0001. (B) Principal component analyses generated using Bray–Curtis dissimilarity matrix depicts similarities or lack thereof between the three groups. (C) Abundances of the reconstructed genomes are depicted for method-3 + EtOH, method-3 + magnetic bead clean-up and method-4 extraction. (D) Heatmap demonstrating the mOTUs for the three methods is depicted. The hierarchical clustering for the heatmap was generated using Ward’s clustering algorithm.